Neuron | 彭勃等人提出了驗證膠質(zhì)細胞轉(zhuǎn)分化的基本原則，并證明NeuroD1不能介導(dǎo)小膠質(zhì)細胞-神經(jīng)元重編程
圖1 論文首頁
圖2 In vivo microglia-specific lineage tracing does not support the microglia-to-neuron conversion.
圖3 Lentivirus induces non-specific labeling in vivo, which may confound the microglia-to-neuron observation.
圖4 Even under microglia depleted brain, the “microglia-converted neurons” are detected, reflecting a lentiviral leakage artifact.
圖5 該研究主要結(jié)論的總結(jié)。
換/移植小膠質(zhì)細胞失控的分子開關(guān)
原文鏈接：
https://www.cell.com/neuron/fulltext/S0896-6273(21)00944-2
附：彭勃課題組2018-2021年發(fā)表的代表性論文
(1) Rao Y.*, Du S., Yang B., Wang Y., Li Y., Li R., Zhou T., Du X., He Y., Wang Y., Zhou X., Yuan T.-F.*, Mao Y.* and Peng B.* (2021) NeuroD1 induces microglial apoptosis and cannot induce microglia-to-neuron cross-lineage reprogramming, Neuron, 109.
(2) Huang Y.#, Xu, Z.#, Xiong S., Sun F., Qin G., Hu G., Wang J., Zhao L., Liang Y.-X., Wu T., Lu Z., Humayun M.S., So K.-F., Pan Y., Li N., Yuan T.-F.*, Rao Y.* and Peng B.* (2018). Repopulated microglia are solely derived from the proliferation of residual microglia after acute depletion. Nature Neuroscience 21, 530-540.
(3) Xu Z.#, Rao Y.#, Huang Y., Zhou T., Feng R., Xiong S., Yuan T.F., Qin S., Lu Y., Zhou X., Li X., Qin B., Mao Y., and Peng B.* (2020). Efficient strategies for microglia replacement in the central nervous system. Cell Reports 32, 108041.
(4) Huang Y.#, Xu Z.#, Xiong S., Qin G., Sun F., Yang J., Yuan T.F., Zhao L., Wang K., Liang Y.X., Fu L., Wu T., Lu Z., So K.F., Rao Y.* and Peng B.* (2018) Dual origins of retinal microglia in the model of microglia repopulation. Cell Discovery 4, 9.
(5) Xu, Z., Zhou, X., Peng, B.*, and Rao, Y.* (2021). Microglia replacement by bone marrow transplantation (Mr BMT) in the central nervous system of adult mice. STAR Protocols 2, 100666.
(6) Xu, Z., Rao, Y.*, and Peng, B.* (2021). Protocol for microglia replacement by peripheral blood (Mr PB). STAR Protocols 2, 100613.
(7) Xu, Z., Peng, B.*, and Rao, Y.* (2021). Microglia replacement by microglia transplantation (Mr MT) in the adult mouse brain. STAR Protocols 2, 100665.
參考文獻
1.Heins, N. et al. Glial cells generate neurons: the role of the transcription factor Pax6. Nature neuroscience 5, 308-315, doi:10.1038/nn828 (2002).
2.Guo, Z. et al. In vivo direct reprogramming of reactive glial cells into functional neurons after brain injury and in an Alzheimer's disease model. Cell stem cell 14, 188-202, doi:10.1016/j.stem.2013.12.001 (2014).
3.Chanda, S. et al. Generation of induced neuronal cells by the single reprogramming factor ASCL1. Stem Cell Reports 3, 282-296, doi:10.1016/j.stemcr.2014.05.020 (2014).
4.Borges, K., McDermott, D., Irier, H., Smith, Y. & Dingledine, R. Degeneration and proliferation of astrocytes in the mouse dentate gyrus after pilocarpine-induced status epilepticus. Exp Neurol 201, 416-427, doi:10.1016/j.expneurol.2006.04.031 (2006).
5.Huang, Y. et al. Repopulated microglia are solely derived from the proliferation of residual microglia after acute depletion. Nature neuroscience 21, 530-540, doi:10.1038/s41593-018-0090-8 (2018).
6.Matsuda, T. et al. Pioneer Factor NeuroD1 Rearranges Transcriptional and Epigenetic Profiles to Execute Microglia-Neuron Conversion. Neuron 101, 472-485 e477, doi:10.1016/j.neuron.2018.12.010 (2019).
7.Wang, L.-L. et al. Revisiting astrocyte to neuron conversion with lineage tracing in vivo. Cell, doi:https://doi.org/10.1016/j.cell.2021.09.005 (2021).
8.Rao, Y. et al. NeuroD1 induces microglial apoptosis and cannot induce microglia-to-neuron cross-lineage reprogramming. Neuron 109 (2021).
9.Xu, Z., Peng, B. & Rao, Y. Microglia replacement by microglia transplantation (Mr MT) in the adult mouse brain. STAR Protoc 2, 100665, doi:10.1016/j.xpro.2021.100665 (2021).
10.Xu, Z., Zhou, X., Peng, B. & Rao, Y. Microglia replacement by bone marrow transplantation (Mr BMT) in the central nervous system of adult mice. STAR Protoc 2, 100666, doi:10.1016/j.xpro.2021.100666 (2021).
11.Xu, Z., Rao, Y. & Peng, B. Protocol for microglia replacement by peripheral blood (Mr PB). STAR Protoc 2, 100613, doi:10.1016/j.xpro.2021.100613 (2021).
12.Xu, Z. et al. Efficient Strategies for Microglia Replacement in the Central Nervous System. Cell reports 32, 108041, doi:10.1016/j.celrep.2020.108041 (2020).
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