Vpack慢病毒包裝系統(tǒng)★ ：
九游會(huì)j9生物新升級(jí)的慢病毒包裝系統(tǒng)，全面解決病毒不出毒和滴度低的問(wèn)題，針對(duì)編碼基因或非編碼基因均有效提高滴度，平均滴度提高十倍以上。在此基礎(chǔ)上改進(jìn)的lncRNA慢病毒新包裝系統(tǒng)，保證LncRNA表達(dá)更精準(zhǔn)，載體熒光表達(dá)更亮，病毒出毒更穩(wěn)定。
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該包裝系統(tǒng)已申請(qǐng)國(guó)家發(fā)明專利，獲得授權(quán)。專利號(hào)為：ZL2020114185140
Vpack系統(tǒng)與常規(guī)病毒包裝系統(tǒng)的比較如下：
九游會(huì)j9新升級(jí)lncRNA慢病毒包裝系統(tǒng)與傳統(tǒng)包裝的方式比較如下：
九游會(huì)j9生物慢病毒系列產(chǎn)品：
定制病毒：涵蓋過(guò)表達(dá)/干擾、CRISPR/Cas9、Cumate誘導(dǎo)表達(dá)系統(tǒng)、Tet-on/off系統(tǒng)、Cre-loxp系統(tǒng)。
現(xiàn)貨慢病毒：涵蓋CRISPR/Cas9敲除或激活文庫(kù)、自噬單標(biāo)或雙標(biāo)、熒光素酶現(xiàn)貨、Cre/Flp重組酶、光遺傳學(xué)等工具類現(xiàn)貨。
慢病毒感染效果圖：
慢病毒能夠有效地感染培養(yǎng)的肝細(xì)胞、心肌細(xì)胞、神經(jīng)元、內(nèi)皮細(xì)胞、干細(xì)胞等多種類型的細(xì)胞。
不同培養(yǎng)體系對(duì)應(yīng)細(xì)胞數(shù)、感染試劑用量及病毒量參考：
儲(chǔ)存液濃度：Polybrene：1ug/μl，Polybrene-plus：100ug/μl
參考工作濃度：Polybrene：5μg/ml，Polybrene-plus：1μg/μl
※細(xì)胞數(shù)量為推薦鋪板細(xì)胞數(shù)量，需根據(jù)細(xì)胞大小調(diào)整鋪板數(shù)量（以感染時(shí)細(xì)胞密度30%為宜），按照實(shí)際感染時(shí)細(xì)胞數(shù)量去計(jì)算病毒加入量。
※為增加病毒與細(xì)胞接觸的幾率，可適當(dāng)減少感染時(shí)培養(yǎng)基的體積。
感染復(fù)數(shù)（Multiplicity of Infection, MOI），含義為感染時(shí)病毒和細(xì)胞數(shù)量的比值，即平均每個(gè)細(xì)胞感染的病毒活性單位數(shù)（慢病毒單位為TU/cell）。
實(shí)驗(yàn)中按照設(shè)置好的MOI計(jì)算每孔需要加入的病毒量：
每孔加病毒量(μl)=MOI×感染時(shí)的細(xì)胞數(shù)/滴度(TU/ml)×1000。
客戶使用九游會(huì)j9慢病毒的文章列表 （部分）
[01]Cell.(IF=41.582). Xu M, Xu HH, Lin Yet al.LECT2,aligand for Tiel, plays a crucial role in liver fibrogenesis[J].2019,178(6):1478-1492.e20.[慢病毒,肝纖維化]
[02]Cell Metabolism.(IF=27.287).Lv H, Lv G, Chen C, et al. NAD+ metabolism maintains inducible PD-L1 expression to drive tumor immune evasion[J].2021,33(1):110-127.e5.[慢病毒過(guò)表達(dá)、干擾,腫瘤免疫,肝癌]
[03]Blood.(IF=22.113).Liu F. Gao Y. Xu B.et al. PEG10 amplification at 7a21.3 potentiates large-cell transformation in cutaneous T-cell lymphoma: PEG10 amplification potentiates LCT in CTCL[J].2021.[慢病毒干擾淋巴瘤]
[04]Nature cell biology.(IF=17.728).Shen SM, Zhang C, Ge MK, et al. PTENα and PTENβ promote carcinogenesis through WDR5 and H3K4 trimethylation[J].2019,21(11):1436-1448.[慢病毒過(guò)表達(dá)、Cas9/dCas9,甲基化肝癌]
[05]Journal of hematology&oncolog.(IF=17.388).Yang L, Chen Y, Liu N, et al. Low expression of TRAF3IP2-AS1 promotes progression of NONO-TFE3 translocation renal cell carcinoma by stimulating N 6-methyladenosine of PARP1 mRNA and downregulating PTEN[J].2021,14(1)1-20.[慢病毒干擾,腎細(xì)胞癌]
[06]Autophagy.(IF=16.016).Wang B, Yin X, Gan W, et al.PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renalcellcarcinoma[Jl.Autophagy.[慢病毒過(guò)表達(dá)，腎細(xì)胞癌]
[07].Cell Death &Differentiation.(IF=15.828)Ji J, Ding K, Luo T, et al.TRIM22 activates NF-kB sienaling in glioblastoma by accelerating the degradation of lκBα[J].2021,28(1):367-381.[慢病毒 過(guò)表達(dá)、Cas9,膠質(zhì)瘤]
[08]Cell Death &Differentiation.(IF=15.828).Hou Y, Zhang O, Pang W, et al.YTHDC1-medated augmentation of mir-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect[J].2021:1-20.[慢病毒過(guò)表達(dá),腫瘤代謝,胰腺癌]
[09]Cell Death &Differentiation.(IF=15.828).Ji J, DingK, LuoT, et al.TRIM22 activates NF-κB signaling in glioblastoma by accelerating the degradation of lκBα[J].2021,28(1): 367-381.[慢病毒過(guò)表達(dá)、Cas9.信號(hào)通路,膠質(zhì)瘤]
[10]Nature communications.(IF=14.919).Song H, Song J, Cheng M, et al.METTL3-mediated m6A RNA methylation promotes the anti-tumour immunity of natural killer cells[J]. 2021,12(1):1-15.[慢病毒干擾,腫瘤免疫,NK細(xì)胞,肝癌]
[11]Nature communications.(IF=14.919).Liu J, Liu ZX, Wu QN, et al.Long noncoding RNA AGPG regulates PFKFB3-mediated tumor glycolytic reprogramming[J].2020,11(1): 1-16.[慢病毒 過(guò)表達(dá) 干擾,糖酵解,食道鱗狀細(xì)胞癌]
[12]Nature communications.(IF=14.919).Ge MK, Zhang N, Xia L, et al.FBX022 degrades nuclear PTEN to promote tumorienesis[J].2020,11(1):1-14.[慢病毒Cas9,結(jié)直腸癌]
[13]Nature communications.(IF=14.919).Zhane M, Yang W, Wang P, et al.CCL7 recruits cDC1 to promote antitumor immunity and facilitate checkpoint immunotherapy to non-small cell lung cancer[J].2020,11(1)1-17.[腺病毒-CRE]
[14]Nature communications.(IF=14.919).Yang Z, Xu G, Wane B, et al.UP12 downregulaion orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade[J].2021,12(1):1-14.[慢病毒 過(guò)表達(dá),腺病毒-crePD-1耐藥]
[15]Nature Communications.(IF=14.919).Huang X, Pan L, Zuo Z, et al.LINC0082 inactivates transcription co-regulatorPGC-1α to promote pancreatic cancer malignancy through metabolic remodelling[J].2021,12(1):1-16[慢病毒Cas9,腫瘤代謝,胰腺癌]
[16] Nature communications.(IF=14.919).Wang Z, Wang F, Zhong J, et al.Using apelin-based synthetic Notch receptors to detect angiogenesis and treat solid tumors[J].2020,11(1)1-13.[慢病毒 過(guò)表達(dá),血管生產(chǎn)]
[17]Theranostics.(IF=11.556).Chen Y, Pan C, Wang X, et al.Silencing of METTL3 effectively hinders invasion and metastasis of prostate cancer cells[J].2021,11(16):7640.[慢病毒干擾,前列腺癌]
[18]Clinical and Translational Medicine(IF311.492).Wang C, Yang Z, Xu E, et al.Anolipoprotein C-Ⅱinduces EMT to promote gastric cancer peritoneal metastasis via PI3K/AKT/mTOR pathway[J].2021,11(8)e522.[慢病毒 過(guò)表達(dá) 干擾,胃癌]
[19]Molecular Therapy.(IF=11.454).Li R, Zeng L, Zhao H, et al.ATXN2-mediated translation of TNFR1 promotes esophageal squamous cell carcinoma via m6A-dependent manner[J].2022.[慢病毒過(guò)表達(dá),食管鱗狀細(xì)胞癌]
[20]Journal of Experimental &Clinical Cancer Research.(IF=11.161).Song C, Zhou C.HOXA10 mediates epithelial-mesenchymal transition to promote gastric cancer metastasis partly via modulation of TGFB2/Smad/METTL3 signaling axis[J].2021,40(1)1-16[慢病毒干擾、過(guò)表達(dá),胃癌]
推薦載體列表（部分）
