腺相關(guān)病毒（Adeno-associated virus，AAV）是一類無法自主復(fù)制、無包膜的二十面體微小病毒，其直徑約20-26nm，含有4.7kb左右的線狀單鏈DNA作為基因組。AAV感染約3周可達(dá)表達(dá)高峰
重組腺相關(guān)病毒載體（recombinant AAV, rAAV）是在非致病的野生型AAV基礎(chǔ)上改造而成的基因載體，研究中采用的rAAV通常為AAV2型基因組與不同的衣殼蛋白結(jié)合產(chǎn)生的組合型重組腺相關(guān)病毒載體，即rAAV2/x，其中x為不同衣殼蛋白的血清型。
自互補AAV（Self-complementary AAV, scAAV）是在rAAV的基礎(chǔ)上將其編碼區(qū)域設(shè)計成雙鏈DNA，感染細(xì)胞后互補部分互補形成雙鏈DNA，不需等待第二鏈DNA合成，因此scAAV感染后3-5天即可到達(dá)表達(dá)峰值。
九游會j9生物AAV產(chǎn)品優(yōu)勢
純度高：
＞95% 純度 內(nèi)毒素低：
＜10 EU/mL 內(nèi)毒素
周期短：
10個工作日 病毒包裝 空殼率低：
＜5% 空殼率
感染效果
九游會j9生物可提供
定制rAAV：
10,000+病毒載體庫，涵蓋過表達(dá)/干擾、CRISPR/Cas9、Cre-loxp系統(tǒng)、Tet-on/off系統(tǒng)、特異性表達(dá)系統(tǒng)。
現(xiàn)貨rAAV：
1,500+現(xiàn)貨病毒載體庫，涵蓋光遺傳學(xué)、化學(xué)遺傳學(xué)、鈣/電壓指示劑、神經(jīng)遞質(zhì)探針、Cre/Flp重組酶等工具類AAV，滿足您從示蹤標(biāo)記、生理調(diào)控到觀察驗證的全面靈活服務(wù)需求。
rAAV載體常用血清型列表
※注：以上AAV基因型均為2型。九游會j9生物新血清型AAV發(fā)現(xiàn)系統(tǒng)AAVneO
TM持續(xù)發(fā)現(xiàn)新型AAV，請關(guān)注九游會j9生物公眾號。
常見啟動子列表
九游會j9生物有60余種特異性啟動子，涉及神經(jīng)系統(tǒng)、心血管、肝臟、肌肉、肺、脂肪、胰腺、眼等研究領(lǐng)域，滿足基礎(chǔ)科研實驗需求。
部分客戶文章列表
[01] Nature medicine. (IF=53.440). Zhu L J, Li T Y, Luo C X, et al. CAPON-nNOS coupling can serve as a target for developing new anxiolytics[J]. 2014, 20(9): 1050-1054. [AAV, 焦慮]
[02] Nature medicine. (IF=53.440). Cao X, Li L P, Wang Q, et al. Astrocyte-derived ATP modulates depressive-like behaviors[J]. 2013, 19(6): 773-777. [AAV, 抑郁]
[03] Nature. (IF=49.962). Yang Y, Cui Y, Sang K, et al. Ketamine blocks bursting in the lateral habenula to rapidly relieve depression[J]. 2018, 554(7692): 317-322. [AAV, 抑郁癥]
[04] Science. (IF=47.728). Ren S, Wang Y, Yue F, et al. The paraventricular thalamus is a critical thalamic area for wakefulness[J]. 2018, 362(6413): 429-434. [AAV, 覺醒]
[05] Science. (IF=47.728). Mu D, Deng J, Liu K F, et al. A central neural circuit for itch sensation[J]. 2017, 357(6352): 695-699. [AAV, 癢覺]
[06] Science. (IF=47.728). Zhou T, Zhu H, Fan Z, et al. History of winning remodels thalamo-PFC circuit to reinforce social dominance[J]. 2017, 357(6347): 162-168. [AAV, 社會行為]
[07] Science. (IF=47.728). Liu D, Gu X, Zhu J, et al. Medial prefrontal activity during delay period contributes to learning of a working memory task[J]. 2014, 346(6208): 458-463. [AAV, 學(xué)習(xí)記憶]
[08] Science. (IF=47.728). Li K, Zhou T, Liao L, et al. βCaMKII in lateral habenula mediates core symptoms of depression[J]. 2013, 341(6149): 1016-1020. [AAV, 抑郁]
[09] Cell. (IF=41.582). Zhu H, Wang N, Yao L, et al. Moderate UV exposure enhances learning and memory by promoting a novel glutamate biosynthetic pathway in the brain[J]. 2018, 173(7): 1716-1727. e17. [AAV, 學(xué)習(xí)記憶]
[10] Cell. (IF=41.582). Bian W J, Miao W Y, He S J, et al. Coordinated spine pruning and maturation mediated by inter-spine competition for cadherin/catenin complexes[J]. 2015, 162(4): 808-822. [AAV, 神經(jīng)環(huán)路]
[11] Cell. (IF=41.582). Wu H, Yu Y, Huang H, et al. Progressive pulmonary fibrosis is caused by elevated mechanical tension on alveolar stem cells[J]. 2020, 180(1): 107-121. e17. [AAV, 呼吸系統(tǒng)]
[12] Circulation. (IF=29.69).Gao P, You M, Li L, et al. Salt-Induced Hepatic Inflammatory Memory Contributes to Cardiovascular Damage Through Epigenetic Modulation of SIRT3[J]. 2022 Feb;145(5):375-391. [AAV, 肝臟]
[13] Cell Metabolism. (IF=27.287). Lu K, Shi TS, Shen SY,et al. Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progression[J]. 2022 Mar 1;34(3):441-457.e7. [AAV, 肝骨軸]
[14] Cell Metabolism. (IF=27.287). Ji Y X, Wang Y, Li P L, et al. A kinome screen reveals that Nemo-like kinase is a key suppressor of hepatic gluconeogenesis[J]. 2021, 33(6): 1171-1186. e9. [AAV、ADV, 肝臟]
[15] Cell Metabolism. (IF=27.287). Li Y, Xu J, Lu Y, et al. DRAK2 aggravates nonalcoholic fatty liver disease progression through SRSF6-associated RNA alternative splicing[J]. 2021, 33(10): 2004-2020. e9. [AAV, 肝臟]
[16] Cell Metabolism. (IF=27.287). Xiao Y Z , Yang M , Xiao Y , et al. Reducing Hypothalamic Stem Cell Senescence Protects against Aging-Associated Physiological Decline[J]. 2020, 31(3):534-548.e5. [AAV，衰老]
[17] Cell Research. (IF=25.617). Gu X, Hu X D, Wang D Q, et al. Treatment of autosomal recessive hearing loss via in vivo CRISPR/Cas9-mediated optimized homology-directed repair in mice [J]. 2022, 0:1–4. [AAV, 遺傳性耳聾]
[18] Cell Research. (IF=25.617). Liu Z, Le Q, Lv Y, et al. A distinct D1-MSN subpopulation down-regulates dopamine to promote negative emotional state[J]. 2021: 1-18. [AAV，獎賞/厭惡情緒]
[19] Cell Research. (IF=25.617). Xie C, Zhang Y P, Song L, et al. Genome editing with CRISPR/Cas9 in postnatal mice corrects PRKAG2 cardiac syndrome[J]. 2016, 26(10): 1099-1111. [AAV, 心臟]
[20] Cell Research. (IF=25.617). Ma M, Xiong W, Hu F, et al. A novel pathway regulates social hierarchy via lncRNA AtLAS and postsynaptic synapsin IIb[J]. 2020, 30(2): 105-118. [AAV，社會等級]
